Journal: Cellular and molecular gastroenterology and hepatology

Article Title: ANKRD22 Drives Rapid Proliferation of Lgr5 + Cells and Acts as a Promising Therapeutic Target in Gastric Mucosal Injury.

doi: 10.1016/j.jcmgh.2021.06.020

Figure Lengend Snippet: Figure 5. ANKRD22 deletion reduces the inflammatory response by inhibiting activation of the macrophages after gastric mucosal injury. (A and B) Ankrd22 knockout decreased the number of CD45þ cells in damaged mouse gastric mucosa detected by FCM. Rat IgG2b was used as an isotype control (n ¼ 4). (C and D) Effect of Ankrd22 knockout on the expression profiles of inflammatory factors in damaged mouse gastric mucosa detected by Luminex assay (n ¼ 4). (E) Ankrd22 knockout reduced IL1a and TNF-a in mouse damaged gastric mucosa detected by ELISA (n ¼ 3). (A–E) Ankrd22þ/þ and Ankrd22-/- mice were examined 24 hours after intragastric administration of EtOH/HCl or saline solution (Ctrl). (F) Expression levels of ANKRD22 in the macro- phages activated by different concentrations of IFN-g. THP-1 and RAW264.7 macrophages were stimulated with 0, 50, 80, or 100 ng/mL IFN-g for 24 hours. Subsequently, the expression of ANKRD22 was determined by qRT-PCR. The data from the 0 ng/mL group were normalized to 1.0. TUBB and Tubb were used as internal references. (G) Expression levels of Ankrd22 in the activated mouse macrophages detected by qRT-PCR. Data of the PBS-treated group (Ctrl) were normalized to 1.0. Tubb was used as an internal reference. (H and I) Determination of IL1a and TNF-a in supernatant of activated macrophages from Ankrd22þ/þ or Ankrd22-/- mice by ELISA. (J and K) Effects of Ankrd22 knockout on the percentages of CD86þ and CD206þ cells in activated Ankrd22þ/þ and Ankrd22-/- mouse macrophages detected by FCM. Rat IgG2b was used as an isotype control (n ¼ 3). (L and M) Determination of IL12 (p70) and IL10 in the supernatant of activated macrophages from Ankrd22þ/þ or Ankrd22-/- mice by ELISA. (E–M) Macrophages were stimulated with 50 ng/mL IFN-g or 100 ng/mL LPS or PBS (Ctrl) for 24 hours. The data were analyzed by the Student t test and are presented as means ± SD. *P < .05, *P < .01, and ***P < .001. APC-A, Allophycocyanin Area; FITC-A, Fluorescein Isothiocyanate Area; FSC-A, Forward Scatter Area.

Article Snippet: According to the manufacturer’s instructions, the cytokine levels of IL1a, TNF-a, IL12, and IL10 in the samples were determined using ELISA kits (Proteintech).

Techniques: Activation Assay, Knock-Out, Control, Expressing, Luminex, Enzyme-linked Immunosorbent Assay, Saline, Quantitative RT-PCR

Journal: Frontiers in physiology

Article Title: Nitric Oxide Synthase Activity Correlates with OGG1 in Ozone-Induced Lung Injury Animal Models.

doi: 10.3389/fphys.2017.00249

Figure Lengend Snippet: FIGURE 1 | The lung injury induced by ozone and treated with L-arginine and L-NAME was assayed by immunohistochemistry (A, scale bar = 100 µM) and ELISA (n = 5, B). All groups were observed at Day 0, Day 4, Day 8, and Day 12 and 4 rats were observed at different time points. **P < 0.01 vs. Day 0 of ozone group; ##P < 0.01 vs. the same time point of ozone group. Arrows represented α-SMA positive staining.

Article Snippet: ELISA were performed on BALF using kits for rat-specific TNFα, TGFβ1, IL10 (Boster, Wuhan, China) and MPO ELISA Kits (CUSABIO, MD, USA), according to the manufacturer’s instructions.

Techniques: Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Staining

Journal: Frontiers in physiology

Article Title: Nitric Oxide Synthase Activity Correlates with OGG1 in Ozone-Induced Lung Injury Animal Models.

doi: 10.3389/fphys.2017.00249

Figure Lengend Snippet: FIGURE 4 | The production of 8-ohdG (A) and OGG1 (B) was assayed by ELISA (n = 5) and the protein expression and distribution of OGG1 were assayed by Western blot (C, n = 4) and immunofluorences (D, scale bar = 50 µM). All groups were observed at Day 0, Day 4, Day 8, and Day 12 and 4 rats were observed at different time points. **P < 0.01 vs. Day 0 of ozone group; ##P < 0.01 vs. ozone group. The distribution of OGG1 was observed at Day 12. Arrows represented OGG1 positive staining.

Article Snippet: ELISA were performed on BALF using kits for rat-specific TNFα, TGFβ1, IL10 (Boster, Wuhan, China) and MPO ELISA Kits (CUSABIO, MD, USA), according to the manufacturer’s instructions.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Staining

Journal: Cell Transplantation

Article Title: Progesterone Enhances the Invasion of Trophoblast Cells by Activating PI3K/AKT Signaling Pathway to Prevent Preeclampsia

doi: 10.1177/09636897221145682

Figure Lengend Snippet: The Basic Information About ELISA Kits.

Article Snippet: Rat IL-10 , CSB-E04595r , 62.5 pg/mL-4000 pg/mL , 15.6 pg/mL , CUSABIO (China).

Techniques: Enzyme-linked Immunosorbent Assay